Radial flow enhances QCM biosensor sensitivity

0585171 - FZÚ 2025 RIV NL eng J - Journal Article
Lynn, Nicholas Scott - Forinová, Michala - Spasovová, Monika - Vaisocherová-Lísalová, Hana - Yudin, Petr
Radial flow enhances QCM biosensor sensitivity.
Sensors and Actuators B - Chemical. Roč. 401, Feb (2024), č. článku 134949. E-ISSN 0925-4005
R&D Projects: GA ČR(CZ) GA22-20012S
Grant - others:AV ČR(CZ) LQ100101902
Program: Prémie Lumina quaeruntur
Institutional support: RVO:68378271
Keywords : biosensor * quartz crystal microbalance * radial flow * microfluidics * diffusion limitations * detection of Escherichia coli
OECD category: Biophysics
Impact factor: 8.4, year: 2022

QCM biosensors are widely used for the detection of a variety of pathogenic microorganisms, and there remains a need for further increases in sensitivity. The majority of QCM biosensors are operated under diffusion-limited conditions that, when used with a standard flow cell, lead to the majority of analyte being captured onto re- gions of the crystal surface that have limited to no mass sensitivity. Here we show that simple changes to a flow cell architecture (reduction of flow cell height) can provide increases in sensing signal. Furthermore, we show that the use of radial flow will lead to analyte capture directly in the sensitive region of a crystal, thus providing dramatic increases in the biosensor’s sensitivity. We examine this problem from both a theoretical and numerical perspective and present a new method by which to consider how changes to a sensing assay will affect sensor sensitivity.
Permanent Link: https://hdl.handle.net/11104/0352918