Solid phase extraction methods for purification of positively charged oligosaccharides and glycans

0577618 - ÚIACH 2024 CZ eng A - Abstract
Smolková, Denisa - Moravcová, Dana - Lavická, Jana
Solid phase extraction methods for purification of positively charged oligosaccharides and glycans.
CECE 2023. 18th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i., 2023. s. 32-32. ISBN 978-80-908154-0-7.
[CECE 2023. International Interdisciplinary Meeting on Bioanalysis /18./. 23.10.2023-25.10.2023, Brno]
R&D Projects: GA ČR(CZ) GA22-00236S
Institutional support: RVO:68081715
Keywords : solid phase extraction * oligosaccharides * glycans
OECD category: Analytical chemistry
https://www.ce-ce.org/user_uploads/CECE_2023/CECE%202023%20Proceedings.pdf

The crucial step for the analysis of oligosaccharides and glycans by capillary electrophoresis (CE) and/or mass spectrometry (CE-MS) is their derivatization by label. The signal of the unreacted label is routinely significantly higher, indicating the need to deplete the unreacted label prior to analysis by CE. The suitability of five different solid-phase extraction sorbents (Spe-ed Amide-2, Chromabond HILIC, HyperSep Diol, Bond Elut PBA, and HyperSep Hypercarb) was tested for the clean-up and concentration of positively labeled maltooligosaccharides from the reaction mixture. Maltooligosaccharides containing four to seven glucose units were labeled by cationic tags (2-aminoethyl)trimethylammonium chloride and (carboxymethyl)trimethylammonium chloride hydrazide (Girard’s reagent T). Purified samples were analyzed by CE with conductivity detection to compare the purification selectivity and efficiency using the tested SPE materials. The amide-based sorbent gave the best results and was used to process the N-linked glycans enzymatically released from the ribonuclease B sample.
Permanent Link: https://hdl.handle.net/11104/0346742