Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis

0575767 - ÚEB 2024 RIV DE eng J - Journal Article
Klodová, Božena - Potěšil, D. - Steinbachová, Lenka - Michailidis, Christos - Lindner, A.C. - Hackenberg, D. - Becker, J. D. - Zdráhal, Z. - Twell, D. - Honys, David
Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis.
Plant Reproduction. Roč. 36, č. 3 (2023), s. 213-241. ISSN 2194-7953. E-ISSN 2194-7961
R&D Projects: GA MŠMT(CZ) LTC18034; GA MŠMT(CZ) EF16_019/0000738; GA MŠMT(CZ) LTAUSA18115; GA MŠMT LTC20028; GA MŠMT LTC20050; GA ČR(CZ) GA21-15856S; GA ČR(CZ) GA19-01723S
Institutional support: RVO:61389030
Keywords : Arabidopsis * Male gametophyte * RNA-seq * Proteome
OECD category: Plant sciences, botany
Impact factor: 3.4, year: 2022
Method of publishing: Open access
https://doi.org/10.1007/s00497-023-00471-w

Sexual reproduction in angiosperms requires the production and delivery of two male gametes by a three-celled haploid male gametophyte. This demands synchronized gene expression in a short developmental window to ensure double fertilization and seed set. While transcriptomic changes in developing pollen are known for Arabidopsis, no studies have integrated RNA and proteomic data in this model. Further, the role of alternative splicing has not been fully addressed, yet post-transcriptional and post-translational regulation may have a key role in gene expression dynamics during microgametogenesis. We have refined and substantially updated global transcriptomic and proteomic changes in developing pollen for two Arabidopsis accessions. Despite the superiority of RNA-seq over microarray-based platforms, we demonstrate high reproducibility and comparability. We identify thousands of long non-coding RNAs as potential regulators of pollen development, hundreds of changes in alternative splicing and provide insight into mRNA translation rate and storage in developing pollen. Our analysis delivers an integrated perspective of gene expression dynamics in developing Arabidopsis pollen and a foundation for studying the role of alternative splicing in this model.
Permanent Link: https://hdl.handle.net/11104/0345493