Non-Rabl chromosome organization in endoreduplicated nuclei of barley embryo and endosperm tissues

0575646 - ÚEB 2024 RIV US eng J - Journal Article
Nowicka, Anna - Ferková, Ľuboslava - Said, Mahmoud - Kováčik, Martin - Zwyrtková, Jana - Baroux, C. - Pečinka, Aleš
Non-Rabl chromosome organization in endoreduplicated nuclei of barley embryo and endosperm tissues.
Journal of Experimental Botany. Roč. 74, č. 8 (2023), s. 2527-2541. ISSN 0022-0957. E-ISSN 1460-2431
R&D Projects: GA ČR(CZ) GA18-12197S; GA ČR(CZ) GA21-02929S; GA MŠMT(CZ) EF16_019/0000827
Institutional support: RVO:61389030
Keywords : Barley * cenh3 * CEREBA repeat * embryo * endoreduplication * endosperm * Hordeum vulgare * nuclear organization * Rabl configuration
OECD category: Plant sciences, botany
Impact factor: 6.9, year: 2022
Method of publishing: Open access
https://doi.org/10.1093/jxb/erad036

Rabl organization is a type of interphase chromosome arrangement with centromeres and telomeres clustering at opposite nuclear poles. Here, we analyzed nuclear morphology and chromosome organization in cycling and endoreduplicated nuclei isolated from embryo and endosperm tissues of developing barley seeds. We show that endoreduplicated nuclei have an irregular shape, less sister chromatid cohesion at 5S rDNA loci, and a reduced amount of centromeric histone CENH3. While the chromosomes of the embryo and endosperm nuclei are initially organized in Rabl configuration, the centromeres and telomeres are intermingled within the nuclear space in the endoreduplicated nuclei with an increasing endoreduplication level. Such a loss of chromosome organization suggests that Rabl configuration is introduced and further reinforced by mitotic divisions in barley cell nuclei in a tissue- and seed age-dependent manner.
Permanent Link: https://hdl.handle.net/11104/0345393