New Labeling Strategies for Glycosylation Analysis by CE/LIF and CE-MS

0573229 - ÚIACH 2024 DE eng A - Abstract
Lavická, Jana - Smolková, Denisa - Čmelík, Richard - Duša, Filip
New Labeling Strategies for Glycosylation Analysis by CE/LIF and CE-MS.
HPLC 2023. Final Program. Frankfurt am Main: Gesellschaft Deutscher Chemiker e.V., 2023 - (Lämmerhofer, M.; Schmitz, O.), č. článku P-SAMP02.
[International Symposium on High-Performance Liquid Phase Separations and Related Techniques (HPLC 2023) /51./. 18.06.2023-22.06.2023, Duesseldorf]
R&D Projects: GA ČR(CZ) GA22-00236S
Institutional support: RVO:68081715
Keywords : CE/LIF * CE-MS * capillary electrophoresis * glycosylation analysis
OECD category: Analytical chemistry

In this work, we present design, synthesis, and application of new fluorescent tags providing rapid labeling kinetics, high fluorescence quantum yields, and significantly enhanced detectability by MS. The newly synthetized fluorescent tags based on pyrene, coumarin and/or BODIPY derivatives with various substituents and modifiable linkers affording tuning of fluorescent and MS properties (preferred wavelength and quantum yield, charge) and specific labeling mechanisms (reductive amination, hydrazine formation, etc.) will be presented in detail and applied for oligosaccharide and glycan analysis by CE/LIF and CE-MS.
Permanent Link: https://hdl.handle.net/11104/0343697