SERS-Tags: Selective Immobilization and Detection of Bacteria by Strain-Specific Antibodies and Surface-Enhanced Raman Scattering

0570271 - ÚPT 2024 RIV CH eng J - Journal Article
Benešová, Markéta - Bernatová, Silvie - Mika, Filip - Pokorná, Zuzana - Ježek, Jan - Šiler, Martin - Samek, Ota - Růžička, F. - Rebrošová, K. - Zemánek, Pavel - Pilát, Zdeněk
SERS-Tags: Selective Immobilization and Detection of Bacteria by Strain-Specific Antibodies and Surface-Enhanced Raman Scattering.
Biosensors. Roč. 13, č. 2 (2023), č. článku 182. E-ISSN 2079-6374
R&D Projects: GA MZd(CZ) NU21-05-00341
Grant - others:AV ČR(CZ) MSM100652101
Program: Program na podporu mezinárodní spolupráce začínajících výzkumných pracovníků
Research Infrastructure: Czech-BioImaging III - 90250
Institutional support: RVO:68081731
Keywords : SERS-tag * Escherichia coli * sandwich immunoassay * single-cell detection
OECD category: Microbiology
Impact factor: 5.4, year: 2022
Method of publishing: Open access
https://www.mdpi.com/2079-6374/13/2/182

Efficient separation and sensitive identification of pathogenic bacterial strains is essential for a prosperous modern society, with direct applications in medical diagnostics, drug discovery, biodefense, and food safety. We developed a fast and reliable method for antibody-based selective immobilization of bacteria from suspension onto a gold-plated glass surface, followed by detection using strain-specific antibodies linked to gold nanoparticles decorated with a reporter molecule. The reporter molecules are subsequently detected by surface-enhanced Raman spectroscopy (SERS). Such a multi-functionalized nanoparticle is called a SERS-tag. The presented procedure uses widely accessible and cheap materials for manufacturing and functionalization of the nanoparticles and the immobilization surfaces. Here, we exemplify the use of the produced SERS-tags for sensitive single-cell detection of opportunistic pathogen Escherichia coli, and we demonstrate the selectivity of our method using two other bacterial strains, Staphylococcus aureus and Serratia marcescens, as negative controls. We believe that the described approach has a potential to inspire the development of novel medical diagnostic tools for rapid identification of bacterial pathogens.
Permanent Link: https://hdl.handle.net/11104/0344482