H4K5 Butyrylation Coexist with Acetylation during Human Spermiogenesis and Are Retained in the Mature Sperm Chromatin

0563716 - BTÚ 2023 RIV CH eng J - Journal Article
de la Iglesia, O. - Jauregi, P. - Jodar, M. - Barrachina, F. - Děd, Lukáš - Mallofre, C. - Rodriguez-Carunchio, L. - Manuel Corral, J. - Lluis Ballesca, J. - Komrsková, Kateřina - Castillo, J. - Oliva, R.
H4K5 Butyrylation Coexist with Acetylation during Human Spermiogenesis and Are Retained in the Mature Sperm Chromatin.
International Journal of Molecular Sciences. Roč. 23, č. 20 (2022), č. článku 12398. E-ISSN 1422-0067
R&D Projects: GA ČR(CZ) GJ20-17403Y; GA ČR(CZ) GC20-20217J; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:86652036
Keywords : butyrylation * acetylation * spermatogenesis * sperm * sperm chromatin * epigenetic regulation
OECD category: Biochemistry and molecular biology
Impact factor: 5.6, year: 2022
Method of publishing: Open access
https://www.mdpi.com/1422-0067/23/20/12398

Male germ cells experience a drastic chromatin remodeling through the nucleo-histone to nucleo-protamine (NH-NP) transition necessary for proper sperm functionality. Post-translational modifications (PTMs) of H4 Lys5, such as acetylation (H4K5ac), play a crucial role in epigenetic control of nucleosome disassembly facilitating protamine incorporation into paternal DNA. It has been shown that butyrylation on the same residue (H4K5bu) participates in temporal regulation of NH-NP transition in mice, delaying the bromodomain testis specific protein (BRDT)-dependent nucleosome disassembly and potentially marking retained nucleosomes. However, no information was available so far on this modification in human sperm. Here, we report a dual behavior of H4K5bu and H4K5ac in human normal spermatogenesis, suggesting a specific role of H4K5bu during spermatid elongation, coexisting with H4K5ac although with different starting points. This pattern is stable under different testicular pathologies, suggesting a highly conserved function of these modifications. Despite a drastic decrease of both PTMs in condensed spermatids, they are retained in ejaculated sperm, with 30% of non-colocalizing nucleosome clusters, which could reflect differential paternal genome retention. Whereas no apparent effect of these PTMs was observed associated with sperm quality, their presence in mature sperm could entail a potential role in the zygote.
Permanent Link: https://hdl.handle.net/11104/0335732