Fluorescence activated cell sorting—A selective tool for plant cell isolation and analysis

0562417 - ÚEB 2023 RIV US eng J - Journal Article
Antoniadi, I. - Skalický, Vladimír - Sun, G. - Ma, W. - Galbraith, D. W. - Novák, Ondřej - Ljung, K.
Fluorescence activated cell sorting—A selective tool for plant cell isolation and analysis.
Cytometry. Part A. Roč. 101, č. 9 (2022), s. 725-736. ISSN 1552-4922. E-ISSN 1552-4930
R&D Projects: GA ČR(CZ) GJ17-21581Y; GA MŠMT(CZ) EF16_019/0000827
Institutional support: RVO:61389030
Keywords : autofluorescence * best practices * plant flow cytometry and sorting * protoplasts * viability and integrity
OECD category: Biochemistry and molecular biology
Impact factor: 3.7, year: 2022
Method of publishing: Open access
https://doi.org/10.1002/cyto.a.24461

Instrumentation for flow cytometry and sorting is designed around the assumption that samples are single-cell suspensions. However, with few exceptions, higher plants comprise complex multicellular tissues and organs, in which the individual cells are held together by shared cell walls. Single-cell suspensions can be obtained through digestion of the cells walls and release of the so-called protoplasts (plants without their cell wall). Here we describe best practices for protoplast preparation, and for analysis through flow cytometry and cell sorting. Finally, the numerous downstream applications involving sorted protoplasts are discussed.
Permanent Link: https://hdl.handle.net/11104/0334733