An Electrochemical Sensor for Detection of Neuroblastoma Markers: Complexation Studies as a Tool for the Selection of a Suitable Receptor for Electrode Coating

0559102 - ÚCHP 2023 RIV DE eng J - Journal Article
Salvadori, Karolína - Páleš, Jakub Marián - Shishkanova, T.V. - Trchová, M. - Fajgar, Radek - Matějka, P. - Cuřínová, Petra
An Electrochemical Sensor for Detection of Neuroblastoma Markers: Complexation Studies as a Tool for the Selection of a Suitable Receptor for Electrode Coating.
ChemPlusChem. Roč. 87, č. 8 (2022), č. článku e202200165. ISSN 2192-6506. E-ISSN 2192-6506
R&D Projects: GA ČR(CZ) GA20-07833S
Institutional support: RVO:67985858
Keywords : biosensors * electropolymerization * host-guest chemistry * neuroblastoma markers
OECD category: Analytical chemistry
Impact factor: 3.4, year: 2022
Method of publishing: Limited access

Homovanillate (HVA) and vanilmandelate (VMA) are recognized markers of diseases, including neuroblastoma. However, their detection in urine represents a challenging task due to the complexity of the matrix. Here, a design, synthesis and thorough investigation of polymerizable urea-based receptors interacting with HVA and VMA are reported. The selection of receptor with the best anion recognition properties for electrode coating is based on 1H NMR and UV-Vis complexation studies. The sensor is prepared by electropolymerization with progress monitoring by cyclic voltammetry. The deposited layer is characterized by IR and scanning electron microscopy. The obtained sensor shows an electrochemical impedance spectroscopy response to VMA with linear range 9.9×10-6 to 1.2×10-3 M and LOD of 3.4x10-6 M. The sensor selectivity was demonstrated by the determination of VMA level in the presence of 16 mM HVA and in artificial urine with and without phosphates, with standard deviations of 0.11, 0.17 and 0.09, respectively.
Permanent Link: https://hdl.handle.net/11104/0332522