Metabolomics of Cerebrospinal Fluid in Multiple Sclerosis Compared With Healthy Controls: A Pilot Study

0558392 - FGÚ 2023 RIV CH eng J - Journal Article
Židó, M. - Kačer, D. - Valeš, Karel - Svobodová, Z. - Zimová, D. - Štětkářová, I.
Metabolomics of Cerebrospinal Fluid in Multiple Sclerosis Compared With Healthy Controls: A Pilot Study.
Frontiers in Neurology. Roč. 13, May 26 (2022), č. článku 874121. ISSN 1664-2295. E-ISSN 1664-2295
Institutional support: RVO:67985823
Keywords : multiple sclerosis * CSF * metabolomics * biomarker * fatty acids * amino acids * arginine
OECD category: Neurosciences (including psychophysiology
Impact factor: 3.4, year: 2022
Method of publishing: Open access
https://doi.org/10.3389/fneur.2022.874121

BackgroundMultiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system (CNS) leading to the loss of myelin and axons. Diagnosis is based on clinical findings, MRI, and analysis of cerebrospinal fluid (CSF). CSF is an ultrafiltrate of plasma and reflects inflammatory processes in the CNS. The aim of this study was to perform metabolomics analysis of CSF in patients after the first attack of MS and healthy controls and try to find new specific analytes for MS including those potentially predicting disease activities at the onset. MethodsWe collected CSF from 19 patients (16 females, aged 19-55 years) after the first attack of clinical symptoms who fulfilled revised McDonald criteria of MS and CSF of 19 controls (16 females, aged 19-50 years). Analyses of CSF samples were provided using the high-performance liquid chromatography system coupled with a mass spectrometer with a high-resolution detector (TripleTOF 5600, AB Sciex, Canada). ResultsApproximately 130 selected analytes were identified, and 30 of them were verified. During the targeted analysis, a significant decrease in arginine and histidine and a less significant decrease in the levels of asparagine, leucine/isoleucine, and tryptophan, together with a significant increase of palmitic acid in the patient group, were found. ConclusionWe observed significant differences in amino and fatty acids in the CSF of newly diagnosed patients with MS in comparison with controls. The most significant changes were observed in levels of arginine, histidine, and palmitic acid that may predict inflammatory disease activity. Further studies are necessary to support these findings as potential biomarkers of MS.
Permanent Link: http://hdl.handle.net/11104/0332206