Fabrication of monolithic capillary columns with inner diameter 50-530 mu m employing a mixture of pentaerythritol tetraacrylate and polyhedral oligomeric silsesquioxane-methacrylate as crosslinkers

0556696 - ÚIACH 2023 RIV DE eng J - Journal Article
Planeta, Josef - Moravcová, Dana - Karásek, Pavel - Roth, Michal
Fabrication of monolithic capillary columns with inner diameter 50-530 mu m employing a mixture of pentaerythritol tetraacrylate and polyhedral oligomeric silsesquioxane-methacrylate as crosslinkers.
Journal of Separation Science. Roč. 45, č. 17 (2022), s. 3256-3263. ISSN 1615-9306. E-ISSN 1615-9314
Institutional support: RVO:68081715
Keywords : liquid-chromatography * inverse size-exclusion chromatography * monoliths
OECD category: Analytical chemistry
Impact factor: 3.1, year: 2022
Method of publishing: Limited access

Highly crosslinked monolithic capillary columns with inner diameters in the range of 50-530 mu m were prepared by radical polymerization of pentaerythritol tetraacrylate, polyhedral oligomeric silsesquioxane-methacrylate, and n-octadecyl methacrylate in the presence of methanol, dodecyl alcohol, and polyethylene glycol lauryl ether. Columns were evaluated by inverse size-exclusion chromatography employing a set of polystyrene standards of narrow molecular-size distribution and by scanning electron microscopy. Chromatographic performance under reversed-phase conditions was also evaluated. The combination of two effective crosslinkers as pentaerythritol tetraacrylate and polyhedral oligomeric silsesquioxane-methacrylate in the polymerization mixture allows for the preparation of robust and efficient monolithic capillary columns within a fairly wide range of internal diameters.
Permanent Link: http://hdl.handle.net/11104/0330816