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Self-assembly, drug encapsulation, and cellular uptake of block and gradient copolymers of 2-methyl-2-oxazine and 2-n-propyl/butyl-2-oxazoline

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    0549608 - ÚMCH 2022 RIV US eng J - Journal Article
    Babuka, David - Kolouchová, Kristýna - Loukotová, Lenka - Sedláček, O. - Groborz, Ondřej - Škarková, A. - Zhigunov, Alexander - Pavlova, Ewa - Hoogenboom, R. - Hrubý, Martin - Štěpánek, Petr
    Self-assembly, drug encapsulation, and cellular uptake of block and gradient copolymers of 2-methyl-2-oxazine and 2-n-propyl/butyl-2-oxazoline.
    Macromolecules. Roč. 54, č. 23 (2021), s. 10667-10681. ISSN 0024-9297. E-ISSN 1520-5835
    R&D Projects: GA ČR(CZ) GA19-01602S
    Grant - others: AV ČR(CZ) FWO-19-03
    Program: Bilaterální spolupráce
    Institutional support: RVO:61389013
    Keywords : polyoxazoline * gradient polymer * block polymer
    OECD category: Polymer science
    Impact factor: 6.057, year: 2021
    Method of publishing: Limited access
    https://pubs.acs.org/doi/10.1021/acs.macromol.1c01794

    Self-assembled amphiphilic polymers have been extensively studied for various biomedical applications, as they show advantageous properties for diagnosis and therapy. In this work, we extensively compared amphiphilic copolymers of the hydrophilic monomer 2-methyl-2-oxazine (MeOzi) and the thermoresponsive or hydrophobic monomers 2-propyl-2-oxazoline (PrOx) or 2-butyl-2-oxazoline (BuOx) in both block and gradient monomer distributions. Such a head-to-head comparison between block and gradient copolymers, which has thus far been mostly missing in the available literature, should provide important insight into the differences and similarities between these two architectures. We investigated the properties of our polymers using a wide array of analytical methods, including dynamic light scattering (DLS), small-angle neutron (SANS) and X-ray scattering (SAXS), one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) spectroscopy, transmission electron microscopy (TEM), drug loading (DL), cellular uptake, and cytotoxicity studies. Most of the studied polymers formed self-assembled nanoparticles, but their properties varied with the monomer ratio, polymer length, and polymer architecture, and these factors could be used to fine-tune the properties of the polymer to meet the demands of the desired application. Both block and gradient copolymers showed similar critical association concentrations and DL properties for the antituberculosis drug rifampicin. Finally, we confirmed that the nanoparticles could be internalized by macrophages, which indicates great potential for the utilization of these nanoparticles in drug delivery.
    Permanent Link: http://hdl.handle.net/11104/0326197

     
     
Number of the records: 1