Upconversion nanoparticles as labels for histopathological tissue evaluation

0548556 - ÚIACH 2022 FR eng A - Abstract
Mickert, M. J. - Farka, Z. - Krawczyka, K. - Hlaváček, Antonín - Brandmeier, J. C. - Andersson-Engels, S. - Sjögren, A. - Skládal, P. - Gorris, H. H.
Upconversion nanoparticles as labels for histopathological tissue evaluation.
UPCONline 2021 – Conference program. 2021. s. 56-56.
[Conference on Properties, Design, and Applications of Upconversion Nanomaterials /3./. 06.04.2021-09.04.2021, Zoom Worldwide]
Institutional support: RVO:68081715
Keywords : photon-upconversion * immunohistochemistry * immunocytochemistry
OECD category: Analytical chemistry

In this work, we designed streptavidin-modified UCNPs (SA-UCNP) for the detection of the breast cancer biomarker HER2 on the surface of model breast cancer cell lines (Figure 1A-B) and breast cancer tissue (Figure 1C). The SA-UCNP conjugate achieved a significantly higher signal-to-background ratio compared to a conventional labelling using a streptavidin-carboxyfluorescein conjugate under the same experimental conditions [2]. An in-house made UCNP-conjugate from Lumito was used to label HER2 in breast cancer tissue. The imaging was performed by Lumito’s prototype scanner capable of combining UCNP upconversion imaging and haematoxylin brightfield imaging at a 20× magnification.
Permanent Link: http://hdl.handle.net/11104/0324601