Droplet microfluidics with photon-upconversion luminiscence detection: Towards automated single-cell assays

0547462 - ÚIACH 2022 CZ aba A - Abstract
Křivánková, Jana - Hlaváček, Antonín - Foret, František
Droplet microfluidics with photon-upconversion luminiscence detection: Towards automated single-cell assays.
Book of abstracts of the 11th International Conference Analytical Cytometry. Vol. 1st edition. Praha: AMCA, spol. s r.o., 2021. s. 1-1. ISBN 978-80-88214-26-7.
[International Conference Analytical Cytometry /11./. 02.10.2021-05.10.2021, Ostrava]
R&D Projects: GA ČR(CZ) GA21-03156S
Institutional support: RVO:68081715
Keywords : droplet-based microfluidic chip * photon-upconversion nanoparticles * single-molecule detection
OECD category: Analytical chemistry
https://www.conference.csac.cz/Amca-CSAC/media/content/2021/program/Book-of-abstracts-CSAC2021.pdf

Here, a droplet-based microfluidic chip with luminescence photon-upconversion detection was used for indexing the chemical content of microdroplets (Figure 1). The technique is used for the assay of β-glucosidase that hydrolyzes 4-methylumbelliferyl
β-D-glucopyranoside into fluorescent 4-methylumbelliferone. Further, we discuss the applicability of the presented instrumentation for single-cell analysis utilizing either enzymatic or immunochemical methods with capabilities for single-molecule detection.
Permanent Link: http://hdl.handle.net/11104/0323688