Metabolomics atlas of oral 13C-glucose tolerance test in mice

0547418 - FGÚ 2022 RIV US eng J - Journal Article
Lopes, Magno - Brejchová, Kristýna - Riečan, Martin - Nováková, Michaela - Rossmeisl, Martin - Čajka, Tomáš - Kuda, Ondřej
Metabolomics atlas of oral 13C-glucose tolerance test in mice.
Cell Reports. Roč. 37, č. 2 (2021), č. článku 109833. ISSN 2211-1247. E-ISSN 2211-1247
R&D Projects: GA ČR(CZ) GA20-00317S
Grant - others:AV ČR(CZ) LQ200111901
Program: Prémie Lumina quaeruntur
Research Infrastructure: e-INFRA CZ - 90140
Institutional support: RVO:67985823
Keywords : metabolomics atlas * metabolomics * lipidomics * glucose tolerance * tracer analysis * metabolite cycling * brown adipose tissue * 13C * pathway analysis * de novo lipogenesis
OECD category: Biochemistry and molecular biology
Impact factor: 9.995, year: 2021
Method of publishing: Open access
https://doi.org/10.1016/j.celrep.2021.109833

Glucose tolerance represents a complex phenotype in which many tissues play important roles and interact to regulate metabolic homeostasis. Here, we perform an analysis of 13C6-glucose tissue distribution, which maps the metabolome and lipidome across 12 metabolically relevant mouse organs and plasma, with integrated 13C6-glucose-derived carbon tracing during oral glucose tolerance test (OGTT). We measure time profiles of water-soluble metabolites and lipids and integrate the global metabolite response into metabolic pathways. During the OGTT, glucose use is turned on with specific kinetics at the organ level, but fasting substrates like β-hydroxybutyrate are switched off in all organs simultaneously. Timeline profiling of 13C-labeled fatty acids and triacylglycerols across tissues suggests that brown adipose tissue may contribute to the circulating fatty acid pool at maximal plasma glucose levels. The GTTAtlas interactive web application serves as a unique resource for the exploration of whole-body glucose metabolism and time profiles of tissue and plasma metabolites during the OGTT.
Permanent Link: http://hdl.handle.net/11104/0323657