Preparation of antibacterial electrospun scaffold for skin cells culture.

0547260 - ÚCHP 2022 RIV CZ eng C - Conference Paper (international conference)
Bajsić, E.G. - Veceric, M. - Zdraveva, E. - Mijović, B. - Grgurić, T.H. - Ujčić, Massimo - Trcin, M.T. - Slivac, I.
Preparation of antibacterial electrospun scaffold for skin cells culture.
NANOCON 2018 - Conference Proceedings. Ostrava: Tanger Ltd., 2019, s. 459-464. ISBN 978-80-87294-89-5.
[Anniversary International Conference on Nanomaterials - Research and Application /10./. Brno (CZ), 17.10.2018-19.10.2018]
Institutional support: RVO:67985858
Keywords : electrospinning * surface modification * titanium dioxide
OECD category: Public and environmental health

In this work the function and application of titanium dioxide as a filler in a composite system polycaprolactone/titanium dioxide (PCL/TiO2) was examined. Titanium dioxide was applied by ultrasonic bath on already electrospun PCL fibrous scaffold treated and non-treated with NaOH. A procedure of surface modification of the electrospun PCL fibrous scaffold was made to enhance the interaction of the surface with the TiO2 particles. The surface modification was performed using NaOH for the formation of carboxyl groups on the fibers' surfaces. The water contact angle was measured by goniometer to prove the change from hydrophobic to hydrophilic polymer surface. SEM was used to study the morphology structure of the electrospun PCL fibrous scaffold before and after NaOH treatment and introduction of TiO2. The content of TiO2 on the electrospun PCL fibrous scaffold was determined by TGA. After NaOH treatment the surface of the electrospun PCL fibrous scaffolds changed from hydrophobic to hydrophilic. SEM micrographs show that with the sonification of 30 min homogeneous TiO2 particles distribution was obtained, while after sonification of 60 min, the TiO2 particles tend to agglomerate. The modification of the scaffold surface with NaOH enhances the adhesion of the TiO2 filler. TG analysis show that longer treatment of the electrospun PCL fibrous scaffolds in the ultrasonic bath gives lower thermal stability. The time of 30 minutes in the ultrasonic bath is optimal to provide sufficient amount of the TiO2 particles on the electrospun PCL fibrous scaffold.
Permanent Link: http://hdl.handle.net/11104/0323538