Production of Fucoxanthin from Phaeodactylum tricornutum Using High Performance Countercurrent Chromatography Retaining Its FOXO3 Nuclear Translocation-Inducing Effect

0546834 - MBÚ 2022 RIV CH eng J - Journal Article
Bárcenas-Pérez, Daniela - Střížek, Antonín - Hrouzek, Pavel - Kopecký, Jan - Barradas, M. - Sierra-Ramirez, A. - Fernandez-Marcos, P. J. - Cheel, José
Production of Fucoxanthin from Phaeodactylum tricornutum Using High Performance Countercurrent Chromatography Retaining Its FOXO3 Nuclear Translocation-Inducing Effect.
Marine Drugs. Roč. 19, č. 9 (2021), č. článku 517. E-ISSN 1660-3397
R&D Projects: GA TA ČR(CZ) TN01000048; GA MŠMT(CZ) LO1416
Institutional support: RVO:61388971
Keywords : centrifugal partition chromatography * undaria-pinnatifida * fucoxanthin * Phaeodactylum tricornutum * high performance countercurrent chromatography (HPCCC) * countercurrent chromatography (CCC) * centrifugal partition chromatography (CPC)
OECD category: Microbiology
Impact factor: 6.085, year: 2021
Method of publishing: Open access
https://www.mdpi.com/1660-3397/19/9/517

Phaeodactylum tricornutum is a rich source of fucoxanthin, a carotenoid with several health benefits. In the present study, high performance countercurrent chromatography (HPCCC) was used to isolate fucoxanthin from an extract of P. tricornutum. A multiple sequential injection HPCCC method was developed combining two elution modes (reverse phase and extrusion). The lower phase of a biphasic solvent system (n-heptane, ethyl acetate, ethanol and water, ratio 5/5/6/3, v/v/v/v) was used as the mobile phase, while the upper phase was the stationary phase. Ten consecutive sample injections (240 mg of extract each) were performed leading to the separation of 38 mg fucoxanthin with purity of 97% and a recovery of 98%. The process throughput was 0.189 g/h, while the efficiency per gram of fucoxanthin was 0.003 g/h. Environmental risk and general process evaluation factors were used for assessment of the developed separation method and compared with existing fucoxanthin liquid-liquid isolation methods. The isolated fucoxanthin retained its well-described ability to induce nuclear translocation of transcription factor FOXO3. Overall, the developed isolation method may represent a useful model to produce biologically active fucoxanthin from diatom biomass.
Permanent Link: http://hdl.handle.net/11104/0323239