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Anhydrobiosis in yeast: role of cortical endoplasmic reticulum protein Ist2 in Saccharomyces cerevisiae cells during dehydration and subsequent rehydration

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    0543905 - FGÚ 2022 RIV NL eng J - Journal Article
    Dauss, E. - Papoušková, Klára - Sychrová, Hana - Rapoport, A.
    Anhydrobiosis in yeast: role of cortical endoplasmic reticulum protein Ist2 in Saccharomyces cerevisiae cells during dehydration and subsequent rehydration.
    Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology. Roč. 114, č. 7 (2021), s. 1069-1077. ISSN 0003-6072. E-ISSN 1572-9699
    R&D Projects: GA MŠk(CZ) LTC20006
    Grant - others: COST (European Cooperation in Science and Technology)(XE) CA18113
    Institutional support: RVO:67985823
    Keywords : anhydrobiosis * dehydration-rehydration * yeast * endoplasmic reticulum * endoplasmic reticulum protein Ist2
    OECD category: Microbiology
    Impact factor: 2.158, year: 2021
    Method of publishing: Limited access
    https://doi.org/10.1007/s10482-021-01578-8

    Two Saccharomyces cerevisiae strains, BY4741 and BY4741-derived strain lacking the IST2 gene (ist2 Delta), were used to characterise the possible role of cortical endoplasmic reticulum (ER) protein Ist2 upon cell dehydration and subsequent rehydration. For the first time, we show that not only protein components of the plasma membrane (PM), but also at least one ER membrane protein (Ist2) play an important role in the maintenance of the viability of yeast cells during dehydration and subsequent rehydration. The low viability of the mutant strain ist2 increment upon dehydration-rehydration stress was related to the lack of Ist2 protein in the ER. We revealed that the PM of ist2 increment strain is not able to completely restore its molecular organisation during reactivation from the state of anhydrobiosis. As the result, the permeability of the PM remains high regardless of the type of reactivation (rapid or gradual rehydration). We conclude that ER protein Ist2 plays an important role in ensuring the stability of molecular organisation and functionality of the PM during dehydration-rehydration stress. These results indicate an important role of ER-PM interactions during cells transition into the state of anhydrobiosis and the subsequent restoration of their physiological activities.
    Permanent Link: http://hdl.handle.net/11104/0321010

     
     
Number of the records: 1