Tissue clearing and imaging methods for cardiovascular development

0542872 - FGÚ 2022 RIV US eng J - Journal Article
Kolesová, Hana - Olejníčková, Veronika - Kvasilová, A. - Gregorovičová, Martina - Sedmera, David
Tissue clearing and imaging methods for cardiovascular development.
iScience. Roč. 24, č. 4 (2021), č. článku 102387. E-ISSN 2589-0042
R&D Projects: GA MŠMT(CZ) LTC17023; GA MŠMT(CZ) LM2015062; GA MŠMT(CZ) EF16_013/0001775; GA ČR(CZ) GA18-03207S; GA ČR(CZ) GA18-03461S; GA ČR(CZ) GA21-03847S
Institutional support: RVO:67985823
Keywords : tissue clearing * imaging * cardiovascular development
OECD category: Cardiac and Cardiovascular systems
Impact factor: 6.107, year: 2021
Method of publishing: Open access
https://doi.org/10.1016/j.isci.2021.102387

Tissue imaging in 3D using visible light is limited and various clearing techniques were developed to increase imaging depth, but none provides universal solution for all tissues at all developmental stages. In this review, we focus on different tissue clearing methods for 3D imaging of heart and vasculature, based on chemical composition (solvent-based, simple immersion, hyperhydration, and hydrogel embedding techniques). We discuss in detail compatibility of various tissue clearing techniques with visualization methods: fluorescence preservation, immunohistochemistry, nuclear staining, and fluorescent dyes vascular perfusion. We also discuss myocardium visualization using autofluorescence, tissue shrinking, and expansion. Then we overview imaging methods used to study cardiovascular system and live imaging. We discuss heart and vessels segmentation methods and image analysis. The review covers the whole process of cardiovascular system 3D imaging, starting from tissue clearing and its compatibility with various visualization methods to the types of imaging methods and resulting image analysis.
Permanent Link: http://hdl.handle.net/11104/0320209