Number of the records: 1
The Gut Microbiota Affects Corticosterone Production in the Murine Small Intestine
- 1.0542413 - FGÚ 2022 RIV CH eng J - Journal Article
Ergang, Peter - Vagnerová, Karla - Hermanová, Petra - Vodička, Martin - Jágr, M. - Šrůtková, Dagmar - Dvořáček, V. - Hudcovic, Tomáš - Pácha, Jiří
The Gut Microbiota Affects Corticosterone Production in the Murine Small Intestine.
International Journal of Molecular Sciences. Roč. 22, č. 8 (2021), č. článku 4229. E-ISSN 1422-0067
R&D Projects: GA ČR(CZ) GA18-02993S; GA ČR(CZ) GA21-10845S
Institutional support: RVO:67985823 ; RVO:61388971
Keywords : glucocorticoids * extra-adrenal steroidogenesis * 11beta-hydroxysteroid dehydrogenase * intestine * microbiome * anti-CD3 antibody
OECD category: Physiology (including cytology); Biochemistry and molecular biology (MBU-M)
Impact factor: 6.208, year: 2021
Method of publishing: Open access
Glucocorticoids (GCs) are hormones that are released in response to stressors and exhibit many activities, including immunomodulatory and anti-inflammatory activities. They are primarily synthesized in the adrenal gland but are also produced in peripheral tissues via regeneration of adrenal 11-oxo metabolites or by de novo synthesis from cholesterol. The present study investigated the influence of the microbiota on de novo steroidogenesis and regeneration of corticosterone in the intestine of germ-free (GF) and specific pathogen-free mice challenged with a physical stressor (anti-CD3 antibody i.p. injection). In the small intestine, acute immune stress resulted in increased mRNA levels of the proinflammatory cytokines IL1 beta, IL6 and Tnf alpha and genes involved in de novo steroidogenesis (Stard3 and Cyp11a1), as well as in regeneration of active GCs from their 11-oxo metabolites (Hsd11b1). GF mice showed a generally reduced transcriptional response to immune stress, which was accompanied by decreased intestinal corticosterone production and reduced expression of the GC-sensitive marker Fkbp5. In contrast, the interaction between stress and the microbiota was not detected at the level of plasma corticosterone or the transcriptional response of adrenal steroidogenic enzymes. The results indicate a differential immune stress-induced intestinal response to proinflammatory stimuli and local corticosterone production driven by the gut microbiota.
Permanent Link: http://hdl.handle.net/11104/0319825
Number of the records: 1