Voltammetric sensing of glycans modified by osmium(VI)ligand complexes. The influence of N-acetyl neuraminic acid

0541880 - BFÚ 2022 RIV GB eng J - Journal Article
Trefulka, Mojmír - Černocká, Hana - Havran, Luděk - Hasoň, Stanislav - Fojt, Lukáš - Ostatná, Veronika
Voltammetric sensing of glycans modified by osmium(VI)ligand complexes. The influence of N-acetyl neuraminic acid.
Electrochimica acta. Roč. 369, č. 2021 (2021), č. článku 137658. ISSN 0013-4686. E-ISSN 1873-3859
R&D Projects: GA ČR(CZ) GA18-18154S
Institutional support: RVO:68081707
Keywords : aberrant sialylation * cancer * osmium * glycosylation * proteins
OECD category: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Impact factor: 7.336, year: 2021
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S001346862032051X?via%3Dihub

A quick and simple way of glycan analysis is combining glycans modification by sixvalent osmium complexes with nitrogenous ligands, particularly with N,N,N,N-tetramethylethylenediamine (Os(VI)tem) and their voltammetric analysis. Os(VI)tem-modified oligosaccharides are reduced at the mercury electrode yielding peaks I-III, representing reduction steps of Os(VI) oxocomplexes. In this work we clarified the individual reduction steps and showed analytical applications for Os(VI)tem modified glycans, including distinguishing sialoglycan regioisomer type or discriminating glycans according to the sialic acid content. Presence or absence of sialic acid was tested in complex glycans as well as in glycoproteins. (C) 2020 Elsevier Ltd. All rights reserved.
Permanent Link: http://hdl.handle.net/11104/0319377