Krill Oil Supplementation Reduces Exacerbated Hepatic Steatosis Induced by Thermoneutral Housing in Mice with Diet-Induced Obesity

0541673 - FGÚ 2022 RIV CH eng J - Journal Article
Sistilli, Gabriella - Kalendová, Veronika - Čajka, Tomáš - Irodenko, Ilaria - Bardová, Kristina - Oseeva, Marina - Žáček, P. - Kroupová, Petra - Horáková, Olga - Lackner, K. - Gastaldelli, A. - Kuda, Ondřej - Kopecký, Jan - Rossmeisl, Martin
Krill Oil Supplementation Reduces Exacerbated Hepatic Steatosis Induced by Thermoneutral Housing in Mice with Diet-Induced Obesity.
Nutrients. Roč. 13, č. 2 (2021), č. článku 437. E-ISSN 2072-6643
R&D Projects: GA ČR(CZ) GA17-11027S
EU Projects: European Commission(XE) 722619 - Foie Gras
Institutional support: RVO:67985823
Keywords : NAFLD * obesity * omega-3 * krill oil * phospholipids * high-fat diet * C57BL/6N mice * thermoneutral temperature
OECD category: Endocrinology and metabolism (including diabetes, hormones)
Impact factor: 6.706, year: 2021
Method of publishing: Open access
https://www.mdpi.com/2072-6643/13/2/437

Preclinical evidence suggests that n-3 fatty acids EPA and DHA (Omega-3) supplemented as phospholipids (PLs) may be more effective than triacylglycerols (TAGs) in reducing hepatic steatosis. To further test the ability of Omega-3 PLs to alleviate liver steatosis, we used a model of exacerbated non-alcoholic fatty liver disease based on high-fat feeding at thermoneutral temperature. Male C57BL/6N mice were fed for 24 weeks a lard-based diet given either alone (LHF) or supplemented with Omega-3 (30 mg/g diet) as PLs (krill oil, w3PL) or TAGs (Epax 3000TG concentrate, w3TG), which had a similar total content of EPA and DHA and their ratio. Substantial levels of TAG accumulation (similar to 250 mg/g) but relatively low inflammation/fibrosis levels were achieved in the livers of control LHF mice. Liver steatosis was reduced by >40% in the omega 3PL but not omega 3TG group, and plasma ALT levels were markedly reduced (by 68%) in omega 3PL mice as well. Krill oil administration also improved hepatic insulin sensitivity, and its effects were associated with high plasma adiponectin levels (150% of LHF mice) along with superior bioavailability of EPA, increased content of alkaloids stachydrine and trigonelline, suppression of lipogenic gene expression, and decreased diacylglycerol levels in the liver. This study reveals that in addition to Omega-3 PLs, other constituents of krill oil, such as alkaloids, may contribute to its strong antisteatotic effects in the liver.
Permanent Link: http://hdl.handle.net/11104/0319203