Circadian Regulation of GluA2 mRNA Processing in the Rat Suprachiasmatic Nucleus and Other Brain Structures

0541672 - FGÚ 2022 RIV US eng J - Journal Article
Míková, H. - Kuchtiak, Viktor - Svobodová, Irena - Spišská, V. - Pačesová, D. - Balík, Aleš - Bendová, Z.
Circadian Regulation of GluA2 mRNA Processing in the Rat Suprachiasmatic Nucleus and Other Brain Structures.
Molecular Neurobiology. Roč. 58, č. 1 (2021), s. 439-449. ISSN 0893-7648. E-ISSN 1559-1182
R&D Projects: GA MŠMT(CZ) LQ1604; GA MŠMT(CZ) ED1.1.00/02.0109; GA MZd(CZ) EF16_025/0007444
Institutional support: RVO:67985823
Keywords : circadian clock * GluA2 subunit * R/G editing * rat * suprachiasmatic nucleus
OECD category: Neurosciences (including psychophysiology
Impact factor: 5.686, year: 2021
Method of publishing: Limited access
https://doi.org/10.1007/s12035-020-02141-8

The mammalian circadian system consists of a major circadian pacemaker located in the suprachiasmatic nucleus (SCN) of the hypothalamus and peripheral clocks in the body, including brain structures. The SCN depends on glutamatergic neurotransmission for transmitting signals from the retina, and it exhibits spontaneous 24-h rhythmicity in neural activity. The aim of this work was to evaluate the degree and circadian rhythmicity of AMPA receptor GluA2 subunit R/G editing and alternative flip/flop splicing in the SCN and other brain structures in Wistar rats. Our data show that the circadian rhythmicity in the SCN's GluA2 mRNA level was highest at dawn, while the circadian rhythm in R/G editing peaked at CT10 and the rhythmic flip varied with the acrophase at the late subjective night. The circadian rhythmicity was confirmed for R/G editing and splicing in the CA3 hippocampal area, and rhythmic variation of the flip isoform was also measured in the olfactory bulbs and cerebellum. The correlations between the R/G editing and alternative flip/flop splicing revealed a structure-dependent direction. In the hippocampus, the edited (G)-form level was positively correlated with the flip variant abundance, in accord with published data, by contrast, in the SCN, the flip variant was in associated more with the unedited (R) form. The edited (G) form and flop isoform also predominated in the retina and cerebellum.
Permanent Link: http://hdl.handle.net/11104/0319202