Gestational and pubertal exposure to low dose of di-(2-ethylhexyl) phthalate impairs sperm quality in adult mice

0541390 - BTÚ 2021 RIV US eng J - Journal Article
Dostálová, Pavla - Žatecká, Eva - Děd, Lukáš - Elzeinová, Fatima - Valášková, Eliška - Kubátová, Alena - Korenková, Vlasta - Langerová, Lucie - Komrsková, Kateřina - Pěknicová, Jana
Gestational and pubertal exposure to low dose of di-(2-ethylhexyl) phthalate impairs sperm quality in adult mice.
Reproductive Toxicology. Roč. 96, SEP 2020 (2020), s. 175-184. ISSN 0890-6238. E-ISSN 1873-1708
R&D Projects: GA ČR(CZ) GA18-11275S; GA ČR(CZ) GJ20-17403Y; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:86652036
Keywords : DEHP * Fertility * Spermatogenesis * Steroidogenesis
OECD category: Reproductive biology (medical aspects to be 3)
Impact factor: 3.143, year: 2020
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S0890623820301738?via%3Dihub

Di-(2-ethylhexyl)-phthalate (DEHP) is a compound widely used as a plasticizer, which can leach from plastics into the environment and thus influence human health. The aim of this study was to analyze whether exposure to an environmentally relevant dose of DEHP during mice fetal development or puberty can cause long-lasting changes detectable month/s after the last exposure. We used a DEHP concentration relevant to a daily human intake of 2.4-3 mu g/kg of body weight/day. CD1 outbred mice were treated either in utero or postnatally during puberty and analyzed in adulthood. Analyzing fertility parameters using morphometric, histologic, genomic and proteomic methods we showed that DEHP exposure leads to decreased sperm concentration and quality, in both experimental groups. Moreover, the changes in anogenital distance, seminal vesicle weight, and testicular gene expression suggest a disturbance of androgen signaling in exposed animals. In conclusion, we hereby present, that the prenatal and pubertal exposure to a low dose of DEHP negatively influenced reproductive endpoints in male mice, and some of the effects were persistent until adulthood.
Permanent Link: http://hdl.handle.net/11104/0318963