Functionalization of polymeric nanofibers using platelets for melanocyte culture

0539763 - ÚEM 2021 RIV CZ eng J - Journal Article
Vocetková, Karolína - Sovková, Věra - Buzgo, Matej - Divín, Radek - Amler, Evžen - Filová, Eva
Functionalization of polymeric nanofibers using platelets for melanocyte culture.
Lékař a technika. Biomedicinské inženýrství a informatika. Roč. 50, č. 1 (2020), s. 16-22. ISSN 0301-5491
R&D Projects: GA MŠMT(CZ) LO1508
Institutional support: RVO:68378041
Keywords : nanofibers * platelets * tissue engineering
OECD category: Biomaterials (as related to medical implants, devices, sensors)
Method of publishing: Open access
https://ojs.cvut.cz/ojs/index.php/CTJ/article/view/6131

Tissue engineering is an interdisciplinary field that uses a combination of cells, suitable biomaterials and bioactive molecules to engineer the desired tissue and restore lost function. These principles have quickly begun to spread to the therapy of multiple diseases, including depigmentation disorders. The most common depigmentation disorder is vitiligo, a disease with deep psychosocial implications. Thanks to their unique properties, electrospun polymeric nanofibers represent a material suitable for tissue engineering applications. Furthermore, they may be functionalized with platelets, cells that contain a wide spectrum of growth factors and chemokines. The aim of this paper was to evaluate the functionalization of polymeric nanofibers with platelets and their effects in melanocyte culture. The scaffolds were visualized using scanning electron microscopy, the metabolic activity and proliferation of melanocytes was determined using MTS assay and dsDNA quantification, respectively. Furthermore, the melanocytes were stained and visualized using confocal microscopy. The acquired data showed that poly-ε-caprolactone functionalized with platelets promoted the viability and proliferation of melanocytes. According to the results, such a functionalized scaffold combining nanofibers and platelets may be suitable for melanocyte culture.
Permanent Link: http://hdl.handle.net/11104/0317461