Functionalizable antifouling coatings as tunable platforms for the stress-driven manipulation of living cell machinery

0538757 - FZÚ 2021 RIV CH eng J - Journal Article
Víšová, Ivana - Smolková, Barbora - Uzhytchak, Mariia - Vrabcová, Markéta - Chafai, Djamel Eddine - Houska, Milan - Pastucha, M. - Skládal, P. - Farka, Z. - Dejneka, Alexandr - Vaisocherová-Lísalová, Hana
Functionalizable antifouling coatings as tunable platforms for the stress-driven manipulation of living cell machinery.
Biomolecules. Roč. 10, č. 8 (2020), s. 1-14, č. článku 1146. E-ISSN 2218-273X
R&D Projects: GA MŠMT(CZ) EF16_019/0000760
Grant - others:OP VVV - SOLID21(XE) CZ.02.1.01/0.0/0.0/16_019/0000760; AV ČR LQ100101902
Program: Prémie Lumina quaeruntur
Institutional support: RVO:68378271
Keywords : zwitterionic material * cell mechanotransduction * cell signaling * functional biointerfaces * antifouling polymer brushes * surface modification
OECD category: Biophysics
Impact factor: 4.879, year: 2020
Method of publishing: Open access

Cells are continuously sensing their microenvironment and subsequently respond to di erent physicochemical cues by the activation or inhibition of di erent signaling pathways. To study a very complex cellular response, it is necessary to diminish background environmental influences and highlight the particular event. However, surface-driven nonspecific interactions of the abundant biomolecules from the environment influence the targeted cell response significantly. Yes-associated protein (YAP) translocation may serve as a marker of human hepatocellular carcinoma (Huh7) cell responses to the extracellular matrix and surface-mediated stresses. Here, we propose a platform of tunable functionable antifouling poly(carboxybetain) (pCB)-based brushes to achieve a molecularly clean background for studying arginine, glycine, and aspartic acid (RGD)-induced YAP-connected mechanotransduction.

Permanent Link: http://hdl.handle.net/11104/0316495