Colloidally stable P(DMA-AGME)-Ale-coated Gd(Tb)F3:Tb3+(Gd3+),Yb3+,Nd3+ nanoparticles as a multimodal contrast agent for down- and upconversion luminescence, magnetic resonance imaging, and computed tomography

0537739 - ÚMCH 2022 RIV CH eng J - Journal Article
Shapoval, Oleksandr - Oleksa, Viktoriia - Šlouf, Miroslav - Lobaz, Volodymyr - Trhlíková, Olga - Filipová, Marcela - Janoušková, Olga - Engstová, Hana - Pankrác, J. - Modrý, A. - Herynek, V. - Ježek, Petr - Šefc, L. - Horák, Daniel
Colloidally stable P(DMA-AGME)-Ale-coated Gd(Tb)F3:Tb3+(Gd3+),Yb3+,Nd3+ nanoparticles as a multimodal contrast agent for down- and upconversion luminescence, magnetic resonance imaging, and computed tomography.
Nanomaterials. Roč. 11, č. 1 (2021), č. článku 230. E-ISSN 2079-4991
R&D Projects: GA ČR(CZ) GA19-00676S; GA MŠMT(CZ) LM2018129; GA MŠMT(CZ) EF16_013/0001775; GA MŠMT(CZ) LTC19032
Research Infrastructure: Czech-BioImaging II - 90129
Institutional support: RVO:61389013 ; RVO:67985823
Keywords : up-conversion luminescence * down-conversion luminescence * colloidal stability
OECD category: Polymer science; Cell biology (FGU-C)
Impact factor: 5.719, year: 2021
Method of publishing: Open access
https://www.mdpi.com/2079-4991/11/1/230

Multimodal imaging, integrating several modalities including down- and up-conversion luminescence, T1- and T2(T2*)-weighted MRI, and CT contrasting in one system, is very promising for improved diagnosis of severe medical disorders. To reach the goal, it is necessary to develop suitable nanoparticles that are highly colloidally stable in biologically relevant media. Here, hydrophilic poly(N,N-dimethylacrylamide-N-acryloylglycine methyl ester)-alendronate-[P(DMA-AGME)-Ale]-coated Gd(Tb)F3:Tb3+(Gd3+),Yb3+,Nd3+ nanoparticles were synthesized by a coprecipitation method in ethylene glycol (EG) followed by coating with the polymer. The particles were tho-roughly characterized by a dynamic light scattering (DLS), transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray energy dispersive spectroscopy (EDAX), selected area electron diffraction (SAED), elemental ana-lysis and fluorescence spectroscopy. Aqueous particle dispersions exhibited excellent colloidal stability in water and physiological buffers. In vitro toxicity assessments suggested no or only mild toxicity of the surface-engineered Gd(Tb)F3:Tb3+(Gd3+),Yb3+,Nd3+ particles in a wide range of concentrations. Internalization of the particles by several types of cells, including HeLa, HF, HepG2, and INS, was confirmed by a down- and up-conversion confocal microscopy. Newly developed particles thus proved to be an efficient contrast agent for fluorescence imaging, T1- and T2(T2*)-weighted magnetic resonance imaging (MRI), and computed tomography (CT).
Permanent Link: http://hdl.handle.net/11104/0317231