Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures

0537260 - FZÚ 2021 RIV GB eng J - Journal Article
Polovinkin, Vitaly - Khakurel, Krishna - Babiak, M. - Angelov, Borislav - Schneider, Bohdan - Dohnálek, Jan - Andreasson, Jakob - Hajdu, Janos
Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures.
Journal of Applied Crystallography. Roč. 53, Dec (2020), s. 1416-1424. ISSN 0021-8898. E-ISSN 1600-5767
R&D Projects: GA MŠMT EF16_019/0000789; GA MŠMT EF15_003/0000447; GA MŠMT LQ1606; GA MŠMT(CZ) LM2015043
Grant - others:OP VVV - ADONIS(XE) CZ.02.1.01/0.0/0.0/16_019/0000789; OP VVV - ELIBIO(XE) CZ.02.1.01/0.0/0.0/15_003/0000447
Research Infrastructure: ELI Beamlines III - 90141
Institutional support: RVO:68378271 ; RVO:86652036
Keywords : electron diffraction * membrane protein crystals grown in meso * lipidic cubic phases * focused ion beam milling * lamella preparation
OECD category: Biophysics; Analytical chemistry (BTO-N)
Impact factor: 3.304, year: 2020
Method of publishing: Open access

Electron crystallography of sub-micrometre-sized 3D protein crystals has emerged recently as a valuable field of structural biology. In meso crystallization methods, utilizing lipidic mesophases, particularly lipidic cubic phases (LCPs), can produce high-quality 3D crystals of membrane proteins (MPs). A major step towards realizing 3D electron crystallography of MP crystals, grown in meso, is to demonstrate electron diffraction from such crystals. The first task is to remove the viscous and sticky lipidic matrix that surrounds the crystals without damaging the crystals. Additionally, the crystals have to be thin enough to let electrons traverse them without significant multiple scattering.

Permanent Link: http://hdl.handle.net/11104/0314994