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Analysis of Bacteriophage-Host Interaction by Raman Tweezers

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    0534715 - ÚPT 2021 RIV US eng J - Journal Article
    Pilát, Zdeněk - Jonáš, Alexandr - Pilátová, J. - Klementová, Tereza - Bernatová, Silvie - Šiler, Martin - Maňka, Tadeáš - Kizovský, Martin - Růžička, F. - Pantůček, R. - Neugebauer, U. - Samek, Ota - Zemánek, Pavel
    Analysis of Bacteriophage-Host Interaction by Raman Tweezers.
    Analytical Chemistry. Roč. 92, č. 18 (2020), s. 12304-12311. ISSN 0003-2700. E-ISSN 1520-6882
    R&D Projects: GA ČR(CZ) GA19-20697S; GA ČR(CZ) GF19-29651L; GA MŠk EF15_003/0000476
    Institutional support: RVO:68081731
    Keywords : staphylococcus-aureus * electron-transport * virus structure * spectroscopy
    Subject RIV: BH - Optics, Masers, Lasers
    OECD category: Optics (including laser optics and quantum optics)
    Impact factor: 6.986, year: 2020
    Method of publishing: Limited access
    https://pubs.acs.org/doi/abs/10.1021/acs.analchem.0c01963

    Bacteriophages, or phages for short, are viruses that replicate in bacteria. The therapeutic and biotechnological potential of phages and their lytic enzymes is of interest for their ability to selectively destroy pathogenic bacteria, including antibiotic-resistant strains. Introduction of phage preparations into medicine, biotechnology, and food industry requires a thorough characterization of phage-host interaction on a molecular level. We employed Raman tweezers to analyze the phage-host interaction of Staphylococcus aureus strain FS159 with a virulent phage JK2 (=812K1/420) of the Myoviridae family and a temperate phage 80 alpha of the Siphoviridae family. We analyzed the timeline of phage-induced molecular changes in infected host cells. We reliably detected the presence of replicating phages in bacterial cells within 5 min after infection. Our results lay the foundations for building a Raman-based diagnostic instrument capable of real-time, in vivo, in situ, nondestructive characterization of the phage-host relationship on the level of individual cells, which has the potential of importantly contributing to the development of phage therapy and enzybiotics.
    Permanent Link: http://hdl.handle.net/11104/0312896

     
     
Number of the records: 1