Differential pulse voltammetric determination of homovanillic acid as a tumor biomarker in human urine after hollow fiber-based liquid-phase microextraction

0532424 - ÚFCH JH 2022 RIV NL eng J - Journal Article
Hrdlička, Vojtěch - Barek, J. - Navrátil, Tomáš
Differential pulse voltammetric determination of homovanillic acid as a tumor biomarker in human urine after hollow fiber-based liquid-phase microextraction.
Talanta. Roč. 221, JAN 2021 (2021), č. článku 121594. ISSN 0039-9140. E-ISSN 1873-3573
R&D Projects: GA ČR(CZ) GA20-01589S
Institutional support: RVO:61388955
Keywords : Liquid phase microextraction * Homovanillic acid * Hollow fiber * Differential pulse voltammetry
OECD category: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Impact factor: 6.556, year: 2021
Method of publishing: Limited access

Novel method for the determination of a tumor marker homovanillic acid (HVA) in human urine was developed. Combination of hollow fiber – based liquid-phase microextraction (HF-LPME) and differential pulse voltammetry (DPV) at a cathodically pre-treated boron doped diamond electrode (BDDE) was applied for these purposes. Optimum conditions were: butyl benzoate as supported liquid membrane (SLM) formed on polypropylene HF, 0.1 mol L−1 HCl as donor phase, 0.1 mol L−1 sodium phosphate buffer of pH 6 as acceptor phase, and 30 min extraction time. HF-LPME-DPV concentration dependence was linear in the range from 1.2 to 100 μmol L−1. Limits of quantification (LOQ) and detection (LOD) were 1.2 and 0.4 μmol L−1, respectively. The applicability of the developed method was verified by analysis of human urine. Standard addition method was used, found HVA concentration was 13.5 ± 1.3 μmol L−1, RSD = 9.3% (n=5).
Permanent Link: http://hdl.handle.net/11104/0310920