Advanced Nano-Bio Technologies for Water and Soil Treatment

0524688 - MBÚ 2021 RIV CH eng M - Monography Chapter
Ševců, A. - Dolinová, I. - Cajthaml, Tomáš - Steinová, J. - Špánek, R.
Tool V: Microbiological Methods for Monitoring nZVI Performance in Groundwater Conditions.
Advanced Nano-Bio Technologies for Water and Soil Treatment. Cham: Springer Nature Switzerland AG, 2020 - (Filip, J.; Cajthaml, T.; Najmanová, P.; Černík, M.; Zbořil, R.), s. 645-657. ISBN 978-3-030-29839-5
R&D Projects: GA TA ČR TE01020218
Institutional support: RVO:61388971
Keywords : microbial monitoring * cultivation * fish * real-time PCR * sequencing
OECD category: Environmental biotechnology
https://link.springer.com/chapter/10.1007/978-3-030-29840-1_34

Biodegradation is a cost-effective tool in the remediation of persistent organic pollutants but can take place only when the concentrations of toxic substances
are decreased to levels suitable for microbial growth, i.e., after nZVI application. Assessment of the impact of nZVI on indigenous microbial communities is, therefore, an essential step in designing a successful remediation strategy. Here, we summarize the most common microbiological methods for monitoring the effect of nZVI on microorganisms such as the cultivation of bacteria, fluorescence in situ hybridization (FISH), phospholipid fatty acid (PLFA) analysis, real-time PCR, and sequencing.


Permanent Link: http://hdl.handle.net/11104/0309007