Mmi1, the Yeast Ortholog of Mammalian Translationally Controlled Tumor Protein (TCTP), Negatively Affects Rapamycin-Induced Autophagy in Post-Diauxic Growth Phase

0524512 - MBÚ 2021 RIV CH eng J - Journal Article
Vojtová, Jana - Hašek, Jiří
Mmi1, the Yeast Ortholog of Mammalian Translationally Controlled Tumor Protein (TCTP), Negatively Affects Rapamycin-Induced Autophagy in Post-Diauxic Growth Phase.
Cells. Roč. 9, č. 1 (2020), č. článku 138. E-ISSN 2073-4409
R&D Projects: GA ČR(CZ) GA16-05497S
Grant - others:U.S.Embassy(CZ) W.Fulbright Commission
Institutional support: RVO:61388971
Keywords : Mmi1 * TCTP * translationally controlled tumor protein
OECD category: Microbiology
Impact factor: 6.600, year: 2020
Method of publishing: Open access
https://www.mdpi.com/2073-4409/9/1/138

Translationally controlled tumor protein (TCTP) is a multifunctional and highly conserved protein from yeast to humans. Recently, its role in non-selective autophagy has been reported with controversial results in mammalian and human cells. Herein we examine the effect of Mmi1, the yeast ortholog of TCTP, on non-selective autophagy in budding yeast Saccharomyces cerevisiae, a well-established model system to monitor autophagy. We induced autophagy by nitrogen starvation or rapamycin addition and measured autophagy by using the Pho8 Delta 60 and GFP-Atg8 processing assays in WT, mmi1 Delta, and in autophagy-deficient strains atg8 Delta or atg1 Delta. Our results demonstrate that Mmi1 does not affect basal or nitrogen starvation-induced autophagy. However, an increased rapamycin-induced autophagy is detected in mmi1 Delta strain when the cells enter the post-diauxic growth phase, and this phenotype can be rescued by inserted wild-type MMI1 gene. Further, the mmi1 Delta cells exhibit significantly lower amounts of reactive oxygen species (ROS) in the post-diauxic growth phase compared to WT cells. In summary, our study suggests that Mmi1 negatively affects rapamycin-induced autophagy in the post-diauxic growth phase and supports the role of Mmi1/TCTP as a negative autophagy regulator in eukaryotic cells.
Permanent Link: http://hdl.handle.net/11104/0308862