0523841 - FGÚ 2021 RIV GB eng J - Journal Article
Deschamps, A. - Colinet, A. S. - Zimmermannová, Olga - Sychrová, Hana - Morsomme, P.
A new pH sensor localized in the Golgi apparatus of Saccharomyces cerevisiae reveals unexpected roles of Vph1p and Stv1p isoforms.
Scientific Reports. Roč. 10, č. 1 (2020), č. článku 1881. ISSN 2045-2322. E-ISSN 2045-2322
R&D Projects: GA ČR(CZ) GA17-01953S
Institutional support: RVO:67985823
Keywords : Golgi * pHluorin * V-ATPase * yeast * STV1 * VPH1 * glucose availability
OECD category: Microbiology
Impact factor: 4.380, year: 2020
Method of publishing: Open access
https://doi.org/10.1038/s41598-020-58795-w

The gradual acidification of the secretory pathway is conserved and extremely important for eukaryotic cells, but until now there was no pH sensor available to monitor the pH of the early Golgi apparatus in Saccharomyces cerevisiae. Therefore, we developed a pHluorin-based sensor for in vivo measurements in the lumen of the Golgi. By using this new tool we show that the cis- and medial-Golgi pH is equal to 6.6-6.7 in wild type cells during exponential phase. As expected, V-ATPase inactivation results in a near neutral Golgi pH. We also uncover that surprisingly Vph1p isoform of the V-ATPase is prevalent to Stv1p for Golgi acidification. Additionally, we observe that during changes of the cytosolic pH, the Golgi pH is kept relatively stable, mainly thanks to the V-ATPase. Eventually, this new probe will allow to better understand the mechanisms involved in the acidification and the pH control within the secretory pathway.
Permanent Link: http://hdl.handle.net/11104/0308119