Flow cytometric characterisation of the complex polyploid genome of Saccharum officinarum and modern sugarcane cultivars

0522098 - ÚEB 2020 RIV GB eng J - Journal Article
Metcalfe, C. J. - Li, J. - Giorgi, D. - Doležel, Jaroslav - Piperidis, N. - Aitken, K. S.
Flow cytometric characterisation of the complex polyploid genome of Saccharum officinarum and modern sugarcane cultivars.
Scientific Reports. Roč. 9, DEC 18 (2019), č. článku 19362. ISSN 2045-2322. E-ISSN 2045-2322
R&D Projects: GA MŠMT(CZ) EF16_019/0000827
Institutional support: RVO:61389030
Keywords : MAP * IDENTIFICATION * CHROMOSOMES
OECD category: Biochemistry and molecular biology
Impact factor: 3.998, year: 2019
Method of publishing: Open access
https://www.nature.com/articles/s41598-019-55652-3

Sugarcane (Saccharum spp.) is a globally important crop for sugar and bioenergy production. Its highly polyploid, complex genome has hindered progress in understanding its molecular structure. Flow cytometric sorting and analysis has been used in other important crops with large genomes to dissect the genome into component chromosomes. Here we present for the first time a method to prepare suspensions of intact sugarcane chromosomes for flow cytometric analysis and sorting. Flow karyotypes were generated for two S. officinarum and three hybrid cultivars. Five main peaks were identified and each genotype had a distinct flow karyotype profile. The flow karyotypes of S. officinarum were sharper and with more discrete peaks than the hybrids, this difference is probably due to the double genome structure of the hybrids. Simple Sequence Repeat (SSR) markers were used to determine that at least one allelic copy of each of the 10 basic chromosomes could be found in each peak for every genotype, except R570, suggesting that the peaks may represent ancestral Saccharum sub genomes. The ability to flow sort Saccharum chromosomes will allow us to isolate and analyse chromosomes of interest and further examine the structure and evolution of the sugarcane genome.
Permanent Link: http://hdl.handle.net/11104/0306600