Restricted and Non-Essential Redundancy of RNAi and piRNA Pathways in Mouse Oocytes

0521819 - ÚMG 2020 RIV US eng J - Journal Article
Táborská, Eliška - Pasulka, Josef - Malík, Radek - Horvat, Filip - Jeníčková, Irena - Matoševič, Z.J. - Svoboda, Petr
Restricted and Non-Essential Redundancy of RNAi and piRNA Pathways in Mouse Oocytes.
PLoS Genetics. Roč. 15, č. 12 (2019), č. článku e1008261. ISSN 1553-7404. E-ISSN 1553-7404
R&D Projects: GA MŠMT LO1419; GA MŠMT(CZ) LM2015040; GA MŠMT ED2.1.00/19.0395; GA MŠMT(CZ) ED1.1.00/02.0109; GA MŠMT(CZ) LM2015062; GA MŠMT(CZ) LM2015040; GA MŠMT ED2.1.00/19.0395
Grant - others:GA MŠk(CZ) LM2015042
Institutional support: RVO:68378050
Keywords : DOUBLE-STRANDED-RNA * GENE-EXPRESSION * RETROTRANSPOSON SUBFAMILY * MAMMALIAN OOCYTES * PIWI PROTEINS MILI * IDENTIFICATION * AMPLIFICATION * TRANSCRIPTION * INTERFERENCE
OECD category: Genetics and heredity (medical genetics to be 3)
Impact factor: 5.175, year: 2019
Method of publishing: Open access
https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1008261

Germline genome defense evolves to recognize and suppress retrotransposons. One of defensive mechanisms is the PIWI-associated RNA (piRNA) pathway, which employs small RNAs for sequence-specific repression. The loss of the piRNA pathway in mice causes male sterility while females remain fertile. Unlike spermatogenic cells, mouse oocytes posses also RNA interference (RNAi), another small RNA pathway capable of retrotransposon suppression. To examine whether RNAi compensates the loss of the piRNA pathway, we produced a new RNAi pathway mutant DicerSOM and crossed it with a catalytically-dead mutant of Mili, an essential piRNA gene. Normal follicular and oocyte development in double mutants showed that RNAi does not suppress a strong ovarian piRNA knock-out phenotype. However, we observed redundant and non-redundant targeting of specific retrotransposon families illustrating stochasticity of recognition and targeting of invading retrotransposons. Intracisternal A Particle retrotransposon was mainly targeted by the piRNA pathway, MaLR and RLTR10 retrotransposons were targeted mainly by RNAi. Double mutants showed accumulations of LINE-1 retrotransposon transcripts. However, we did not find strong evidence for transcriptional activation and mobilization of retrotransposition competent LINE-1 elements suggesting that while both defense pathways are simultaneously expendable for ovarian oocyte development, yet another transcriptional silencing mechanism prevents mobilization of LINE-1 elements.
Permanent Link: http://hdl.handle.net/11104/0306383