Combining NMR Spectroscopy and Molecular Dynamic Simulations to Solve and Analyze the Structure of Protein-RNA Complexes

0520492 - BFÚ 2020 RIV US eng J - Journal Article
Campagne, S. - Krepl, Miroslav - Šponer, Jiří - Allain, F.H.T.
Combining NMR Spectroscopy and Molecular Dynamic Simulations to Solve and Analyze the Structure of Protein-RNA Complexes.
Methods in Enzymology. Methods in Enzymology. Roč. 614, č. 2019 (2019), s. 393-422. ISSN 0076-6879
Institutional support: RVO:68081707
Keywords : nuclear-magnetic-resonance * multidimensional nmr * recognition * assignment * domain
OECD category: Biochemistry and molecular biology
Impact factor: 1.394, year: 2019
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0076687918303598?via%3Dihub

Understanding the RNA binding specificity of protein is of primary interest to decipher their function in the cell. Here, we review the methodology used to solve the structures of protein-RNA complexes using solution-state NMR spectroscopy: from sample preparation to structure calculation procedures. We also describe how molecular dynamics simulations can help providing additional information on the role of key amino acid side chains and of water molecules in protein-RNA recognition.
Permanent Link: http://hdl.handle.net/11104/0305154