NMR metabolomics reveals effects of Cryptosporidium infections on host cell metabolome

0520226 - BC 2020 RIV GB eng J - Journal Article
Miller, C.N. - Panagos, C.G. - Mosedale, W.R.T. - Kváč, Martin - Howard, M.J. - Tsaousis, A.D.
NMR metabolomics reveals effects of Cryptosporidium infections on host cell metabolome.
Gut Pathogens. Roč. 11, APR 3 2019 (2019), č. článku 13. ISSN 1757-4749. E-ISSN 1757-4749
R&D Projects: GA ČR GA15-01090S
Institutional support: RVO:60077344
Keywords : acquired-immunodeficiency-syndrome * energy-metabolism * oxidative stress * parvum * taurine * culture * burden * epidemiology * apicomplexan * excystation * Cryptosporidiosis * nmr * Metabolomics * colo-680n * Taurine
OECD category: Veterinary science
Impact factor: 3.274, year: 2019
Method of publishing: Open access
https://gutpathogens.biomedcentral.com/track/pdf/10.1186/s13099-019-0293-x

BackgroundCryptosporidium is an important gut microbe whose contributions towards infant and immunocompromise patient mortality rates are steadily increasing. Over the last decade, we have seen the development of various tools and methods for studying Cryptosporidium infection and its interactions with their hosts. One area that is sorely overlooked is the effect infection has on host metabolic processes.ResultsUsing a H-1 nuclear magnetic resonance approach to metabolomics, we have explored the nature of the mouse gut metabolome as well as providing the first insight into the metabolome of an infected cell line. Statistical analysis and predictive modelling demonstrated new understandings of the effects of a Cryptosporidium infection, while verifying the presence of known metabolic changes. Of note is the potential contribution of host derived taurine to the diarrhoeal aspects of the disease previously attributed to a solely parasite-based alteration of the gut environment, in addition to other metabolites involved with host cell catabolism.ConclusionThis approach will spearhead our understanding of the Cryptosporidium-host metabolic exchange and provide novel targets for tackling this deadly parasite.
Permanent Link: http://hdl.handle.net/11104/0304916