Spinning disk-based superresolution microscopy for subdiffraction structured illumination imaging of living cells

0517466 - FZÚ 2020 RIV CZ eng J - Journal Article
Lunov, Oleg - Smolková, Barbora - Lynnyková, Anna - Uzhytchak, Mariia - Kubinová, Šárka - Dejneka, Alexandr
Spinning disk-based superresolution microscopy for subdiffraction structured illumination imaging of living cells.
Jemná mechanika a optika. Roč. 64, č. 5 (2019), s. 127-129. ISSN 0447-6441
R&D Projects: GA MŠMT(CZ) LO1409
Institutional support: RVO:68378271
Keywords : superresolution microscopy * structured illumination microscopy (SIM) * lysosomes * mitochondria
OECD category: Biophysics
Method of publishing: Limited access
https://jmo.fzu.cz/sites/jmo.fzu.cz/files/oldweb/2019/2019-05/jmo_19_05_obsah.pdf

Superresolution microscopy enables to see previously hidden details of cellular structures. However, it requires to use high irradiation intensities that may cause artefacts and photodamage of fragile biological samples. Newly developed confocal technique and Olympus super resolution (OSR), that combines spinning disk confocal with structured illumination microscopy, represents a reliable and fast superresolution avoiding photodamage. We demonstrate an OSR microscope platform, that enables subsecond, multicolour data acquisition. It also provides access to subdiffraction structured illumination imaging. We show that OSR allows live-cell experiments without any noticeable cellular damage. OSR system has an improved lateral (∼2) and axial (∼3) resolution compared with conventional confocal imaging. Moreover, OSR is compatible with both fixed and live cell imaging.
Permanent Link: http://hdl.handle.net/11104/0302767