Genomic Organization of Repetitive DNA Elements and Extensive Karyotype Diversity of Silurid Catfishes (Teleostei: Siluriformes): A Comparative Cytogenetic Approach

0508614 - ÚŽFG 2020 RIV CH eng J - Journal Article
Ditcharoen, S. - Bertollo, L.A.C. - Ráb, Petr - Hnátková, E. - Molina, W. F. - Liehr, T. - Tanomtong, A. - Triantaphyllidis, C. - Ozouf-Costaz, C. - Tongnunui, S. - Pengseng, P. - Supiwong, W. - Aroutiounian, R. - de Bello Cioffi, M.
Genomic Organization of Repetitive DNA Elements and Extensive Karyotype Diversity of Silurid Catfishes (Teleostei: Siluriformes): A Comparative Cytogenetic Approach.
International Journal of Molecular Sciences. Roč. 20, JUL 2 (2019), č. článku 3545. E-ISSN 1422-0067
R&D Projects: GA MŠMT EF15_003/0000460
Institutional support: RVO:67985904
Keywords : fish cytotaxonomy * chromosome banding * FISH
OECD category: Genetics and heredity (medical genetics to be 3)
Impact factor: 4.556, year: 2019
Method of publishing: Open access
https://www.mdpi.com/1422-0067/20/14/3545

The catfish family Siluridae contains 107 described species distributed in Asia, but with some distributed in Europe. In this study, karyotypes and other chromosomal characteristics of 15 species from eight genera were examined using conventional and molecular cytogenetic protocols. Our results showed the diploid number (2n) to be highly divergent among species, ranging from 2n = 40 to 92, with the modal frequency comprising 56 to 64 chromosomes. Accordingly, the ratio of uni- and bi-armed chromosomes is also highly variable, thus suggesting extensive chromosomal rearrangements. Only one chromosome pair bearing major rDNA sites occurs in most species, except for Wallago micropogon, Ompok siluroides, and Kryptoterus giminus with two, and Silurichthys phaiosoma with five such pairs. In contrast, chromosomes bearing 5S rDNA sites range from one to as high as nine pairs among the species. Comparative genomic hybridization (CGH) experiments evidenced large genomic divergence, even between congeneric species. As a whole, we conclude that karyotype features and chromosomal diversity of the silurid catfishes are unusually extensive, but parallel some other catfish lineages and primary freshwater fish groups, thus making silurids an important model for investigating the evolutionary dynamics of fish chromosomes.
Permanent Link: http://hdl.handle.net/11104/0299471