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Identification of ability to form biofilm in Candida parapsilosis and Staphylococcus epidermidis by Raman spectroscopy

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    0508215 - ÚPT 2020 RIV GB eng J - Journal Article
    Rebrošová, K. - Šiler, Martin - Samek, Ota - Růžička, F. - Bernatová, Silvie - Ježek, Jan - Zemánek, Pavel - Holá, V.
    Identification of ability to form biofilm in Candida parapsilosis and Staphylococcus epidermidis by Raman spectroscopy.
    Future Microbiology. Roč. 14, č. 6 (2019), s. 509-518. ISSN 1746-0913. E-ISSN 1746-0921
    R&D Projects: GA ČR(CZ) GA15-20645S; GA MŠk(CZ) LO1212; GA MŠk ED0017/01/01
    Institutional support: RVO:68081731
    Keywords : biofilm * Candida parapsilosis * Raman spectroscopy * rapid diagnostics * Staphylococcus epidermidis
    Subject RIV: BH - Optics, Masers, Lasers
    OECD category: Optics (including laser optics and quantum optics)
    Impact factor: 2.907, year: 2019
    Method of publishing: Limited access
    https://www.futuremedicine.com/doi/10.2217/fmb-2018-0297

    Aim: Finding rapid, reliable diagnostic methods is a big challenge in clinical microbiology. Raman spectroscopy is an optical method used for multiple applications in scientific fields including microbiology. This work reports its potential in identifying biofilm positive strains of Candida parapsilosis and Staphylococcus epidermidis. Materials & methods: We tested 54 S. epidermidis strains (23 biofilm positive, 31 negative) and 51 C. parapsilosis strains (27 biofilm positive, 24 negative) from colonies on Mueller-Hinton agar plates, using Raman spectroscopy. Results: The accuracy was 98.9% for C. parapsilosis and 96.1% for S. epidermidis. Conclusion: Themethod showed great potential for identifying biofilm positive bacterial and yeast strains. We suggest that Raman spectroscopy might become a useful aid in clinical diagnostics.
    Permanent Link: http://hdl.handle.net/11104/0299188

     
     
Number of the records: 1