SIZE AS AN IMPORTANT FACTOR IN NANO-TiO2 TOXICITY IN MACROPHAGE-LIKE CELLS

0507375 - ÚEM 2020 RIV CZ eng C - Conference Paper (international conference)
Líbalová, Helena - Sikorová, Jitka - Brzicová, Táňa - Milcová, Alena - Vrbová, Kristýna - Pikal, P. - Topinka, Jan - Rössner ml., Pavel
SIZE AS AN IMPORTANT FACTOR IN NANO-TiO2 TOXICITY IN MACROPHAGE-LIKE CELLS.
Nanocon 2017 : conference proceedings : 9th International Conference on Nanomaterials - Research & Application. Ostrava: Tanger Ltd., 2018, s. 733-738. ISBN 9788087294819.
[International Conference on Nanomaterials - Research and Application (NANOCON) /9./. Brno (CZ), 18.10.2017-20.10.2017]
R&D Projects: GA MŠMT(CZ) LO1508; GA ČR(CZ) GBP503/12/G147; GA MŠMT(CZ) LM2015073
Institutional support: RVO:68378041
Keywords : TiO2 * nanoparticles * macrophages * cytotoxicity
OECD category: Biochemistry and molecular biology

A set of NPs consists of 5 variants of anatase and 5 variants of rutile nanoparticles differing in their diameter (from 3 to 165 nm). TiO2 samples were characterized in the powder form and dispersed in water and cell culture media. Three cytotoxicity assays were used: MTS, WST-1, and LDH. For all nanomaterials, three independent repetitions were carried out.

Overall, cytotoxicity of all NPs was low even at the highest concentration of 256 mu g/ml. The viability of cells did not decrease below 60% for WST-1 and MTS assays and 80% for the LDH assay. Besides concentration, crystalline size was identified as the most important cytotoxic factor. Clear nonlinear relationship between crystalline size and cytotoxicity was detected, higher toxicity induced NPs within the size range 20-60 nm. Increased cytotoxicity in given diameter size range would give an answer to inconsistent findings at size and cytotoxicity relationship.
Permanent Link: http://hdl.handle.net/11104/0302799