Possibility of Using Purine Oxidation Signals for DNA Sequence Analysis

0506713 - BFÚ 2020 RIV CZ eng C - Conference Paper (international conference)
Špaček, Jan - Cahová, Kateřina - Havran, Luděk - Fojta, Miroslav
Possibility of Using Purine Oxidation Signals for DNA Sequence Analysis.
XXXV. Moderní elektrochemické metody. Sborník přednášek mezinárodní odborné konference. Ústí nad Labem: Srsenová Lenka - Best Servis, 2015 - (Navrátil, T.; Fojta, M.; Schwarzová, K.), Č. 2015 (2015), s. 233-235. ISBN 978-80-905221-3-8.
[Moderní elektrochemické metody /35./. Jetřichovice (CZ), 18.05.2015-22.05.2015]
R&D Projects: GA ČR GBP206/12/G151
Institutional support: RVO:68081707
Keywords : DNA oxidation * DNA sequence analysis * Electrochemistry
OECD category: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Signals provided by electrochemical oxidation of single stranding DNA on the surface of pyrolytic graphite electrode, which correspond to the oxidation of adenine and guanine (A and G) are known for over forty years. The mechanism of oxidation of free A and G as well as nucleoside mono-, and triphosphates of these bases is know but the mechanism of oxidation of purines in the DNA still remains unexplained. Ratiometry of A and G signals could be a new tool for DNA sequence analysis, if signals of A and G oxidation obtained from single-stranded DNA adsorbed at the electrode surface are proportional to the number of oxidized As and Gs in studied samples. Our preliminary results suggest that there is a correlation, yet we encountered problems, which yet has to be addressed and explained before this method could be applied for DNA analysis.
Permanent Link: http://hdl.handle.net/11104/0297905