Constant Current Chronopotentiometry as a Method for Detection of Singlet Oxygen Protein Damage

0506684 - BFÚ 2020 RIV CZ eng C - Conference Paper (international conference)
Ostatná, Veronika - Vargová, Veronika - Černocká, Hana - Paleček, Emil
Constant Current Chronopotentiometry as a Method for Detection of Singlet Oxygen Protein Damage.
XXXVI. Moderní elektrochemické metody. Sborník přednášek. Ústí nad Labem: Best servis, 2016 - (Navrátil, T.; Fojta, M.; Schwarzová, K.), Č. 2016 (2016), s. 158-160. ISBN 978-80-905221-4-5.
[Moderní elektrochemické metody /36./. Jetřichovice (CZ), 23.05.2016-27.05.2016]
R&D Projects: GA ČR GA13-00956S
Institutional support: RVO:68081707
Keywords : carbon electrodes * affinity tags * mercury * purification
OECD category: Physical chemistry

Proteins are one of the major targets for oxidative damage in the cell.Indirect non-radical oxidation of the protein via formation and subsequent reaction with single oxygen (O-1(2))is one of the major processes.In this work we studied the single oxygen(O-1(2))-mediated oxidation of bovine serum albumin (BSA) by constant current chronopotentiometry in combination with mercury electrode.Our chronopotentiometric data show that photo-oxidized BSA was more susceptible to the electric field-induced denaturation than non-oxidized native BSA. Our method utilizing intrinsic electrochemical signal of proteins provides a sensitive detection methods for minor damages in various proteins.
Permanent Link: http://hdl.handle.net/11104/0297882