Inhibition of E. coli growth by nanodiamond and graphene oxide enhanced by Luria-Bertani medium

0500694 - FZÚ 2019 RIV CH eng J - Journal Article
Jíra, Jaroslav - Rezek, B. - Kříha, V. - Artemenko, Anna - Matolínová, I. - Skakalová, V. - Štenclová, Pavla - Kromka, Alexander
Inhibition of E. coli growth by nanodiamond and graphene oxide enhanced by Luria-Bertani medium.
Nanomaterials. Roč. 8, č. 3 (2018), s. 1-13, č. článku 140. E-ISSN 2079-4991
R&D Projects: GA ČR GA15-01687S
Institutional support: RVO:68378271
Keywords : nanodiamonds * graphene oxide * Escherichia coli * antibacterial activity * inhibition
OECD category: Biophysics
Impact factor: 4.034, year: 2018

Nanodiamonds (NDs) and graphene oxide (GO) are modern carbon-based nanomaterials with promising features for the inhibition of microorganism growth ability. Here we compare the effects of nanodiamond and graphene oxide in both annealed and reduced forms in two types of cultivation media-Luria-Bertani (LB) and Mueller-Hinton (MH) broths. The comparison shows that the number of colony forming unit of Escherichia coli is significantly lowered (45%) by all the nanomaterials in LB medium for at least 24 h against control. On the contrary, a significant long-term inhibition of E. coli growth (by 45%) in the MH medium is provided only by hydrogenated NDs terminated with C-HX groups. The specific role of the ND and GO on the enhancement of the oxidative stress of bacteria, therefore isolating them from both the environment was suggested. Analyses by infrared spectroscopy, photoelectron spectroscopy, scanning electron microscopy and dynamic light scattering corroborate these conclusions.
Permanent Link: http://hdl.handle.net/11104/0295494