Identification of COS markers specific for Thinopyrum elongatum chromosomes preliminary revealed high level of macrosyntenic relationship between the wheat and Th. Elongatum genomes

0499503 - ÚEB 2019 RIV US eng J - Journal Article
Gaál, E. - Valárik, Miroslav - Molnár, István - Farkas, A. - Linc, G.
Identification of COS markers specific for Thinopyrum elongatum chromosomes preliminary revealed high level of macrosyntenic relationship between the wheat and Th. Elongatum genomes.
PLoS ONE. Roč. 13, č. 12 (2018), č. článku e0208840. ISSN 1932-6203. E-ISSN 1932-6203
Institutional support: RVO:61389030
Keywords : TRANSLOCATIONS CONFERRING RESISTANCE * MOLECULAR CYTOGENETIC ANALYSIS * HEAD BLIGHT RESISTANCE
OECD category: Genetics and heredity (medical genetics to be 3)
Impact factor: 2.776, year: 2018

Thinopyrum elongatum (Host) D.R. Dewey has served as an important gene source for wheat breeding improvement for many years. The exact characterization of its chromosomes is important for the detailed analysis of prebreeding materials produced with this species. The major aim of this study was to identify and characterize new molecular markers to be used for the rapid analysis of E genome chromatin in wheat background. Sixty of the 169 conserved orthologous set (COS) markers tested on diverse wheat-Th. elongatum disomic/ ditelosomic addition lines were assigned to various Th. elongatum chromosomes and will be used for marker-assisted selection. The macrosyntenic relationship between the wheat and Th. elongatum genomes was investigated using EST sequences. Several rearrangements were revealed in homoeologous chromosome groups 2, 5, 6 and 7, while chromosomes 1 and 4 were conserved. Molecular cytogenetic and marker analysis showed the presence of rearranged chromosome involved in 6ES and 2EL arms in the 6E disomic addition line. The selected chromosome arm-specific COS markers will make it possible to identify gene introgressions in breeding programmes and will also be useful in the development of new chromosome-specific markers, evolutionary analysis and gene mapping.
Permanent Link: http://hdl.handle.net/11104/0291693