Surface-enhanced Raman spectroscopy of chloroalkanes in microfluidic chips

0498741 - ÚPT 2019 RIV US eng C - Conference Paper (international conference)
Pilát, Zdeněk - Kizovský, Martin - Ježek, Jan - Krátký, Stanislav - Sobota, Jaroslav - Šiler, Martin - Samek, Ota - Buryška, T. - Vaňáček, P. - Damborský, J. - Prokop, Z. - Zemánek, Pavel
Surface-enhanced Raman spectroscopy of chloroalkanes in microfluidic chips.
21st Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics. (Proceedings of SPIE 10976). Bellingham: SPIE, 2018 - (Zemánek, P.), č. článku 1097619. ISBN 9781510626072. ISSN 0277-786X.
[Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics /21./. Lednice (CZ), 03.09.2018-07.09.2018]
R&D Projects: GA ČR(CZ) GA16-07965S; GA MŠMT(CZ) LO1212
Institutional support: RVO:68081731
Keywords : Surface enhanced Raman spectroscopy * microfluidics * Klarite 312 * chloroalkane * 1,2,3-trichloropropane
OECD category: Optics (including laser optics and quantum optics)

Optofluidics, a research discipline combining optics and microfluidics, currently aspires to revolutionize the analysis of biological and chemical samples, e.g. for medicine, pharmacology, or molecular biology. In order to detect low concentrations of analytes in water, we have developed an optofluidic device containing a nanostructured substrate for surface enhanced Raman spectroscopy (SERS). The geometry of the gold surface allows localized plasmon oscillations to give rise to the SERS effect, in which the Raman spectral lines are intensified by the interaction of the plasmonic field with the electrons in the molecular bonds. The SERS substrate was enclosed in a microfluidic system, which allowed
transport and precise mixing of the analyzed fluids, while preventing contamination or abrasion of the highly sensitive substrate. To illustrate its practical use, we employed the device for quantitative detection of persistent environmental pollutant 1,2,3-trichloropropane in water in submillimolar concentrations. The developed sensor allows fast and simple quantification of halogenated compounds and it will contribute towards the environmental monitoring and enzymology experiments with engineered haloalkane dehalogenase enzymes.
Permanent Link: http://hdl.handle.net/11104/0291099