Determination of Three Tumor Biomarkers (Homovanillic Acid, Vanillylmandelic Acid, and 5‐Hydroxyindole‐3‐Acetic Acid) Using Flow Injection Analysis with Amperometric Detection

0498457 - ÚFCH JH 2020 RIV DE eng J - Journal Article
Němečková-Makrlíková, Anna - Matysik, F.-M. - Navrátil, Tomáš - Barek, J. - Vyskočil, V.
Determination of Three Tumor Biomarkers (Homovanillic Acid, Vanillylmandelic Acid, and 5‐Hydroxyindole‐3‐Acetic Acid) Using Flow Injection Analysis with Amperometric Detection.
Electroanalysis. Roč. 31, č. 2 (2019), s. 303-308. ISSN 1040-0397. E-ISSN 1521-4109
R&D Projects: GA ČR GA17-03868S
Institutional support: RVO:61388955
Keywords : screen-printed carbon electrodes * flow injection analysis * amerometric detection
OECD category: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Impact factor: 2.544, year: 2019
Method of publishing: Limited access

Flow injection analysis with amperometric detection (FIA‐AD) at screen‐printed carbon electrodes (SPCEs) in optimum medium of Britton‐Robinson buffer (0.04 mol ⋅ L−1, pH 2.0) was used for the determination of three tumor biomarkers (homovanillic acid (HVA), vanillylmandelic acid (VMA), and 5‐hydroxyindole‐3‐acetic acid (5‐HIAA)). Dependences of the peak current on the concentration of biomarkers were linear in the whole tested concentration range from 0.05 to 100 μmol ⋅ L−1, with limits of detection (LODs) of 0.065 μmol ⋅ L−1 for HVA, 0.053 μmol ⋅ L−1 for VMA, and 0.033 μmol ⋅ L−1 for 5‐HIAA (calculated from peak heights), and 0.024 μmol ⋅ L−1 for HVA, 0.020 μmol ⋅ L−1 for VMA, and 0.012 μmol ⋅ L−1 for 5‐HIAA (calculated from peak areas), respectively.

Permanent Link: http://hdl.handle.net/11104/0290822