Profiling system for skin kallikrein proteolysis applied in gene-deficient mouse models

0492934 - ÚOCHB 2019 RIV DE eng J - Journal Article
Horn, Martin - Žbodáková, Olga - Kašpárek, Petr - Srp, Jaroslav - Hanečková, Radka - Hradilek, Martin - Mareš, Michael - Sedláček, Radislav
Profiling system for skin kallikrein proteolysis applied in gene-deficient mouse models.
Biological Chemistry. Roč. 399, č. 9 (2018), s. 1085-1089. ISSN 1431-6730. E-ISSN 1437-4315
R&D Projects: GA MŠMT LO1302; GA MŠMT(CZ) LM2015040; GA MŠMT(CZ) LQ1604; GA MŠMT ED2.1.00/19.0395
Institutional support: RVO:61388963 ; RVO:68378050
Keywords : inhibitors * kallikreins * mouse model * proteolysis * skin * substrates
OECD category: Biochemistry and molecular biology
Impact factor: 3.014, year: 2018

Kallikrein-related proteases (KLKs) play a critical role in epidermis physiology and have been implicated in skin pathologies such as Netherton syndrome. The contribution of individual KLKs to skin proteolysis is poorly understood. Monitoring of their activities in skin proteome is hampered by overlapping substrate specificities, and there is a need for novel assays. Here, we present a platform of selective and sensitive fluorogenic substrates and inhibitors for profiling KLK5, KLK7 and KLK14. These chemical tools were evaluated using recombinant KLKs and tissue from a unique set of mice deficient in eight combinations of KLKs and their natural regulator LEKTI.
Permanent Link: http://hdl.handle.net/11104/0286406