Nanodiamonds as „artificial proteins“: Regulation of a cell signalling system using low nanomolar solutions of inorganic nanocrystals

0492357 - ÚŽFG 2019 RIV GB eng J - Journal Article
Bálek, L. - Buchtová, Marcela - Kunová Bosáková, M. - Vařecha, M. - Foldynová-Trantírková, Silvie - Gudernová, I. - Veselá, I. - Havlík, Jan - Neburková, Jitka - Turner, S. - Krzyscik, M. A. - Zakrzewska, M. - Klimaschewski, L. - Claus, P. - Trantírek, L. - Cígler, Petr - Krejčí, Pavel
Nanodiamonds as „artificial proteins“: Regulation of a cell signalling system using low nanomolar solutions of inorganic nanocrystals.
Biomaterials. Roč. 176, SEP (2018), s. 106-121. ISSN 0142-9612. E-ISSN 1878-5905
R&D Projects: GA MŠMT EF15_003/0000477; GA ČR(CZ) GA17-09525S; GA ČR GA17-12075S; GA MŠMT(CZ) EF16_019/0000729
Institutional support: RVO:67985904 ; RVO:68081707 ; RVO:61388963
Keywords : nanodiamonds * cell signalling * FGF * fibroblast growth factor * nanotherapeutics
OECD category: Nano-materials (production and properties); Nano-materials (production and properties) (BFU-R)
Impact factor: 10.273, year: 2018

The blocking of specific protein-protein interactions using nanoparticles is an emerging alternative to small molecule-based therapeutic interventions. However, the nanoparticles designed as „artificial proteins“ generally require modification of their surface with (bio)organic molecules and/or polymers to ensure their selectivity and specificity of action. Here, we show that nanosized diamond crystals (nanodiamonds, NDs) without any synthetically installed (bio)organic interface enable the specific and efficient targeting of the family of extracellular signalling molecules known as fibroblast growth factors (FGFs). We found that low nanomolar solutions of detonation NDs with positive-potential strongly associate with multiple FGF ligands present at sub-nanomolar concentrations and effectively neutralize the effects of FGF signalling in cells without interfering with other growth factor systems and serum proteins unrelated to FGFs. We identified an evolutionarily conserved FGF recognition motif, 17 amino acids long, that contributes to the selectivity of the ND-FGF interaction. In addition, we inserted this motif into a de novo constructed chimeric protein, which significantly improved its interaction with NDs. We demonstrated that the interaction of NDs, as purely inorganic nanoparticles, with proteins can mitigate pathological FGF signalling and promote the restoration of cartilage growth in a mouse limb explant model. Based on our observations, we foresee that NDs may potentially be applied as nano therapeutics to neutralize disease-related activities of FGFs in vivo.
Permanent Link: http://hdl.handle.net/11104/0285915