Consequences of gut microbiota manipulation by antibiotics in animal model of early immune dysregulation: phenotype analysis of behaviour and biochemical correlates

0492121 - ÚI 2019 GB eng A - Abstract
Tejkalová, H. - Kačer, P. - Klaschka, Jan - Mrázek, Jakub - Kvasnová, Simona - Fliegerová, Kateřina
Consequences of gut microbiota manipulation by antibiotics in animal model of early immune dysregulation: phenotype analysis of behaviour and biochemical correlates.
FEBS Open Bio. Wiley. Roč. 8, Suppl. 1 (2018), s. 199-199. ISSN 2211-5463. E-ISSN 2211-5463
R&D Projects: GA ČR(CZ) GBP304/12/G069; GA MZd(CZ) NV17-31852A
Grant - others:GA MŠk(CZ) LO1611
Institutional support: RVO:67985807 ; RVO:67985904
Keywords : gut microbiome * microbiome * rats * antibiotics * behaviour * neurotransmitters
OECD category: Psychiatry; Microbiology (UZFG-Y)

Host­-microbe interactions are now thought as essential for maintaining host health. Additionally, microbiota and activation of host´s immune system play significant role in the development and regulation of CNS. Furthermore, it was presented that certain microbial proteins can mimic several key neuropeptides involved in the regulation of behaviour. The role of the gut microbiome in the bi­directional interaction on the gut­brain axis and the role in a set time­frame for normal synaptogenesis in the development seem crucial. Finally, infectional insult during perinatal period may be responsible for the development of neuropsychiatric disorders such as schizophrenia in later life. According to these facts, our study was aimed at describing behavioural and biochemical alterations in rats exposed to early inflammatory stimulation. This was based on the subchronic administration of lipopolysaccharide to neonatal rat together with effect of microbiota modulation realised by antibiotic cocktail. The10­days modulation of gut microbiota was started in adults at PD 60 in drinking water. The microbiota from faeces was analysed before and at the end of experiment together with analysis of neurotransmitters from plasma and brain tissues. Shift in the composition of the bacterial diversity in faeces of studied animals was preliminary assessed by DGGE analysis and then determined by the Ion Torrent next­generation sequencing technology. To evaluate changes on low­ and high­molecular level, the collected biological samples were analysed using advanced metabolomic and proteomic strategies. Significant changes in the fingerprints of bioactive molecules among the tested groups were found. The values were discussed with relation to results obtained in behavioural tests, where impairment of behavioural profiles (locomotion and sensorimotor gating) and weight gain deficit were documented.

Permanent Link: http://hdl.handle.net/11104/0285684